Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser
Document Type
Syllabus
Publication Date
1-1-2022
Abstract
Laser microsurgery allows the user to ablate cell bodies or disconnect nerve fibers by using a laser microbeam focused through a microscope. This technique was pioneered in C. elegans where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in C. elegans can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we describe how to assemble and operate a platform for Yb-doped fiber laser microsurgery. The novel laser setup described here is a more robust, lower cost, and user-friendly alternative to other femtosecond-pulsed laser systems.
Identifier
85126923070 (Scopus)
Publication Title
Methods in Molecular Biology
External Full Text Location
https://doi.org/10.1007/978-1-0716-2181-3_17
e-ISSN
19406029
ISSN
10643745
PubMed ID
35320573
First Page
319
Last Page
328
Volume
2468
Recommended Citation
Harreguy, Maria B.; Tran, Tracy S.; and Haspel, Gal, "Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser" (2022). Faculty Publications. 3213.
https://digitalcommons.njit.edu/fac_pubs/3213
