Highly stable phospholipid unilamellar vesicles from spontaneous vesiculation: A DLS and SANS study

Document Type

Article

Publication Date

1-13-2005

Abstract

Spontaneously formed unilamellar vesicles (ULV) composed of short- and long-chain phospholipids, dihexanoyl phosphorylcholine (DHPC) and dimyristoyl phosphorylcholine (DMPC), respectively, were doped with a negatively charged lipid, dimyristoyl phosphorylglycerol (DMPG), and studied with small-angle neutron scattering (SANS) and dynamic light scattering (DLS). Upon dilution, the spontaneous formation of vesicles was found to take place from bilayered micelles, or so-called "bicelles". SANS and DLS data show that ULV with narrow size distributions are highly stable at low lipid (C lp < 0.50 wt %) and NaCl salt (C s) concentrations. ULV size was found to be independent of both C lp and C s when they were below 0.33 and 0.5 wt %, respectively. Surface charge and salinity were found to be important factors in preparing ULV of a certain size. This observation is not in complete agreement with previous experimental results and cannot be completely explained with current theoretical predictions based on equilibrium calculations for catanionic surfactant mixtures. ULV size is found to be invariant over a wide range of temperatures, both below and above the phase-transition temperature, T M, of DMPC, and was stable for periods of weeks and months, even after sonication.

Identifier

12344309271 (Scopus)

Publication Title

Journal of Physical Chemistry B

External Full Text Location

https://doi.org/10.1021/jp047510q

ISSN

15206106

PubMed ID

16851053

First Page

609

Last Page

616

Issue

1

Volume

109

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