Fluorescence activated cell sorting for enzymatic activity

Document Type

Article

Publication Date

5-1-2006

Abstract

Directed evolution is a reliable method for protein engineering and as a tool for investigating structure/function relationships. A key for a successful directed evolution experiment is oftentimes the screen. Fluorescence activated cell sorting (FACS) is powerful high-throughput screening approach to isolate and identify mutants from large protein libraries. FACS has been successful in isolating proteins with improved or altered binding affinity. However, FACS screening for mutants with enhanced catalytic activity has been met with limited success. This review focuses on the FACS screening of protein libraries for enzymatic activity. © 2006 Bentham Science Publishers Ltd.

Identifier

33744496842 (Scopus)

Publication Title

Combinatorial Chemistry and High Throughput Screening

External Full Text Location

https://doi.org/10.2174/138620706776843200

ISSN

13862073

PubMed ID

16724923

First Page

321

Last Page

328

Issue

4

Volume

9

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