A DNA aptamer sensor for 8-oxo-7,8-dihydroguanine

Document Type

Article

Publication Date

1-15-2012

Abstract

Abasic site-containing DNA duplex is a versatile structural motif that can be used for the design of purine aptamers and sensors. In this study, several modifications were introduced to the abasic site to explore possible specific binding of free 8-oxoG, a product of DNA base excision repair. The nucleoside opposite the abasic site was replaced by pyrrolo-dC as a reporter group. Binding of 8-oxoG quenched pyrrolo-dC fluorescence by as much as 70%. In contrast, adenine, guanine, thymine, and cytosine showed only minimal fluorescence quenching effect. The best aptamer binds 8-oxoG with a dissociation constant of 5.5 ± 0.8 μM. This sensor can be used to accurately measure 8-oxoG concentrations in the presence of guanine. © 2011 Elsevier Ltd. All rights reserved.

Identifier

84855660329 (Scopus)

Publication Title

Bioorganic and Medicinal Chemistry Letters

External Full Text Location

https://doi.org/10.1016/j.bmcl.2011.12.046

e-ISSN

14643405

ISSN

0960894X

PubMed ID

22209455

First Page

863

Last Page

867

Issue

2

Volume

22

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