New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling
Document Type
Conference Proceeding
Publication Date
4-16-2012
Abstract
New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
Identifier
84859612320 (Scopus)
ISBN
[9780819488763]
Publication Title
Progress in Biomedical Optics and Imaging Proceedings of SPIE
External Full Text Location
https://doi.org/10.1117/12.921056
ISSN
16057422
Volume
8233
Recommended Citation
Pillai, Shyamala; Wirpsza, Laura; Kozlov, Maxim; Marras, Salvatore A.E.; Krasnoperov, Lev N.; and Mustaev, Arkady, "New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling" (2012). Faculty Publications. 18289.
https://digitalcommons.njit.edu/fac_pubs/18289
