Complex optically computed phase microscopy for label-free sub-cellular imaging

Document Type

Article

Publication Date

4-15-2023

Abstract

In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.

Identifier

85167685336 (Scopus)

Publication Title

Optics Continuum

External Full Text Location

https://doi.org/10.1364/OPTCON.486396

e-ISSN

27700208

First Page

793

Last Page

800

Issue

4

Volume

2

Grant

1R15GM148990-01

Fund Ref

National Institute of General Medical Sciences

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