In vitro synthesis of nitroxide free radicals by hog liver microsomes

Document Type

Article

Publication Date

1-1-1990

Abstract

The in vitro biooxidation of 4-hydroxy-2,2,6,60tetramethylpiperidine (TEMP), 4-hydroxy-2,2,4,4-tetyramethyl-1,3-oxazolidine (TEMO) and diphenylamine (DPA)_by hog liver microsomes to their respective nitroxide free radicals, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), 2,2,4,4-tetramethyl-1,3-oxazolidine-1-oxyl (TEMOO), and diphenylnitroxide (DPNO) has been investigated. For extending the life span of the liver microsomes, a calcium alginate immobilization procedure was used. The biooxidation rates of the above amines to their respective nitroxide metabolites were measured by means of oxygen uptake at 37°C and pH 7.4. N-octylamine was found to be an activator in the biooxidation of the amines. The formation of the nitroxide radicals was identified by E.S.R. spectroscopy. © 1990.

Identifier

0025678791 (Scopus)

Publication Title

Free Radical Biology and Medicine

External Full Text Location

https://doi.org/10.1016/0891-5849(90)90009-8

ISSN

08915849

PubMed ID

2178149

First Page

345

Last Page

353

Issue

4

Volume

9

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