Use of Ca+2 to re-aggregate lipopolysaccharide (LPS) in hemoglobin solutions and the subsequent removal of endotoxin by ultrafiltration

Authors

Liping Li, Newark College of Engineering
Robert G. Luo, Newark College of Engineering

Document Type

Article

Publication Date

1-1-1998

Abstract

Divalent cations such as Ca⁺² can act as "bridges" between lipopolysaccharide subunits causing large vesicles to form. During a two-stage ultrafiltration Ca⁺² re-aggregated lipopolysaccharide subunits in protein solutions into large vesicles that could not pass a 300,000 nominal molecular weight cut-off ultrafiltration membrane. Such re-aggregation occurred at concentration ratio of hemoglobin Ao / lipopolysaccharide equal to or less than 17.0, or at concentration ratio of Ca⁺² / hemoglobin Ao / lipopolysaccharide equal to or greater than 8.5. © 1998 Chapman & Hall.

Identifier

0031885348 (Scopus)

Publication Title

Biotechnology Techniques

External Full Text Location

https://doi.org/10.1023/a:1008884332586

ISSN

0951208X

First Page

119

Last Page

122

Issue

2

Volume

12

Grant

421280

Fund Ref

Colgate-Palmolive Company

Recommended Citation

Li, Liping and Luo, Robert G., "Use of Ca+2 to re-aggregate lipopolysaccharide (LPS) in hemoglobin solutions and the subsequent removal of endotoxin by ultrafiltration" (1998). Faculty Publications. 16473.
https://digitalcommons.njit.edu/fac_pubs/16473