Elastomeric platform with surface wrinkling patterns to control cardiac cell alignment

Document Type

Article

Publication Date

8-1-2023

Abstract

There is a growing interest in creating 2D cardiac tissue models that display native extracellular matrix (ECM) cues of the heart tissue. Cellular alignment alone is known to be a crucial cue for cardiac tissue development by regulating cell–cell and cell-ECM interactions. In this study, we report a simple and robust approach to create lamellar surface wrinkling patterns enabling spatial control of pattern dimensions with a wide range of pattern amplitude (A ≈ 2–55 μm) and wavelength (λ ≈ 35–100 μm). For human cardiomyocytes (hCMs) and human cardiac fibroblasts (hCFs), our results indicate that the degree of cellular alignment and pattern recognition are correlated with pattern A and λ. We also demonstrate fabrication of devices composed of micro-well arrays with user-defined lamellar patterns on the bottom surface of each well for high-throughput screening studies. Results from a screening study indicate that cellular alignment is strongly diminished with increasing seeding density. In another study, we show our ability to vary hCM/hCF seeding ratio for each well to create co-culture systems where seeding ratio is independent of cellular alignment.

Identifier

85147579313 (Scopus)

Publication Title

Journal of Biomedical Materials Research Part A

External Full Text Location

https://doi.org/10.1002/jbm.a.37511

e-ISSN

15524965

ISSN

15493296

PubMed ID

36762538

First Page

1228

Last Page

1242

Issue

8

Volume

111

Fund Ref

New Jersey Health Foundation

This document is currently not available here.

Share

COinS