In vitro investigation of streptokinase activity using sonoclot coagulation analysis

Document Type

Conference Proceeding

Publication Date

1-1-2003

Abstract

Streptoklnase (SK) therapy has become a common clinical intervention in the treatment of acute coronary syndromes. Recent clinical trials indicate that adjuvant treatment with low molecular weight heparin (LMWH) or bivalirudin (BV) may attenuate fibrinolytic-induced procoagulant activity. We investigated the effects of SK with LMWH and BV on the clotting of whole blood using a Sonoclot® Coagulation Analyzer. Human citrated whole blood was spiked with SK and LMWH at final concentrations of control, 5 U/mL SK, 0.2 U/mL LMWH, and 5 U/mL SK:0.2 U/mL LMWH. The samples (n=10) were incubated at 37°C for 10 minutes and recalcified with CaCl2 to initiate clotting. Tracings were obtained using the Sonoclot Signature Viewer software, and the clotting time (CT) and maximum clot viscosity were noted. The experiment was repeated (n=8) using 5 μg/mL BV in place of LMWH. The Sonoclot tracings provide a whole-picture analysis of the effects of SK and adjuvant anticoagulants on haemostatic and fibrinolytic mechanisms. Implementation of coagulation monitoring protocols for SK therapy may aid in clinical diagnosis.

Identifier

84943377177 (Scopus)

Publication Title

Proceedings of the IEEE Annual Northeast Bioengineering Conference Nebec

e-ISSN

21607001

ISSN

1071121X

First Page

253

Last Page

254

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