Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation

Document Type

Article

Publication Date

7-20-2003

Abstract

Hollow fiber membranes and chromatographic resin beads are commonly employed in a variety of bioseparation processes. A new class of integrated separation devices is being studied in which the shell side of a hollow fiber device is filled with adsorbents/ chromatographic resin beads. Such devices and the corresponding separation methods integrate feed broth clarification by the microfiltration/ultrafiltration membrane with bioproduct purification by the shell-side resin beads either as an adsorbent or as beads in elution chromatography. A mathematical model has been developed for the prediction of the chromatographic behavior of such an integrated device. Simulations have been done to study the effects of axial dispersion, feed flow rate, water permeation rate, fiber packing density, and void fraction. Numerical solutions were obtained by solving the governing equations. This model can reasonably describe the concentration profiles as well as the breakthrough and elution behaviors in the integrated device. © 2003 Wiley Periodicals, Inc.

Identifier

0038377222 (Scopus)

Publication Title

Biotechnology and Bioengineering

External Full Text Location

https://doi.org/10.1002/bit.10653

ISSN

00063592

PubMed ID

12768618

First Page

125

Last Page

139

Issue

2

Volume

83

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