Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

Document Type

Article

Publication Date

10-18-2011

Abstract

Here we developed a microcontact printing (µCP) process to directly pattern enzymes in a single step without the loss of enzyme activity after printing. By modifying the substrate to display aldehyde groups, the direct stamping of urease enables the simultaneous patterning and covalent cross-linking of urease under the reducing agent NaCNBH4, which does not degrade the enzyme activity. Because the enzyme was not treated by the cross-linker prior to the sampling but rather pre-assembled on the surface of the substrate, only the contact areas of the PDMS stamp reacted with the cross-linker on the substrate, minimizing the poisoning of the enzymatic sites. The exposed urease particles on the substrate, free from the cross-linker, were still catalytically active and utilized to grow crystalline ZnO nanoparticles on the enzyme patterns in ambient conditions and in aqueous solution. © The Royal Society of Chemistry.

Identifier

80054993643 (Scopus)

Publication Title

Journal of Materials Chemistry

External Full Text Location

https://doi.org/10.1039/c1jm11609f

e-ISSN

13645501

ISSN

09599428

First Page

16877

Last Page

16879

Issue

42

Volume

21

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