Synthesis of PLGA–lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG–lipid nanoparticles for siRNA delivery
Document Type
Syllabus
Publication Date
1-1-2017
Abstract
The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.
Identifier
85029378955 (Scopus)
Publication Title
Methods in Molecular Biology
External Full Text Location
https://doi.org/10.1007/978-1-4939-7138-1_15
ISSN
10643745
PubMed ID
28730443
First Page
231
Last Page
240
Volume
1632
Recommended Citation
Wang, Lei; Griffel, Benjamin; and Xu, Xiaoyang, "Synthesis of PLGA–lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG–lipid nanoparticles for siRNA delivery" (2017). Faculty Publications. 10017.
https://digitalcommons.njit.edu/fac_pubs/10017
