Date of Award

Spring 1982

Document Type


Degree Name

Doctor of Engineering Science in Chemical Engineering


Chemical Engineering and Chemistry

First Advisor

Ching-Rong Huang

Second Advisor

John E. McCormick

Third Advisor

David S. Kristol

Fourth Advisor

Edward Charles Roche, Jr.

Fifth Advisor

Rong-Yaw Chen


A new semi-continuous parametric pumping process for the separation of protein mixtures has been developed, based on cyclic variation of pH and electric field. The model system used for this process consisted of a mixture of human serum albumin and human hemoglobin in contact with CM Sepharose cation exchanger. Experimental results show that the protein separation via the pH parapump with electric field is two to three times greater than the results which are obtained in batch chromatography or via either single-column or multi-column parapumps with cyclic variation of pH and ionic strength.