Date of Award
Doctor of Engineering Science in Chemical Engineering
Chemical Engineering and Chemistry
John E. McCormick
David S. Kristol
Edward Charles Roche, Jr.
A new semi-continuous parametric pumping process for the separation of protein mixtures has been developed, based on cyclic variation of pH and electric field. The model system used for this process consisted of a mixture of human serum albumin and human hemoglobin in contact with CM Sepharose cation exchanger. Experimental results show that the protein separation via the pH parapump with electric field is two to three times greater than the results which are obtained in batch chromatography or via either single-column or multi-column parapumps with cyclic variation of pH and ionic strength.
Hollein, Helen Conway, "Separation of proteins via pH parametric pumping with electric field" (1982). Dissertations. 1273.