Date of Award
Doctor of Engineering Science in Chemical Engineering
Chemical Engineering and Chemistry
Edward Charles Roche, Jr.
Mahmoud F. Abd-El-Bary
Dimitrios P. Tassios
The separation of protein mixtures via pH-parametric pumping was investigated both theoretically and experimentally. A simple system consisting of one column packed either with cation or anion exchanger was first considered. A system of parapumps was then developed with more columns which were connected in series and packed alternately with cation and anion exchangers. Various methods of operation of parapumps are discussed. Enrichment and spliting of protein mixtures were examined. In most cases, the separation factor was defined at a steady state condition and was improved by increasing the number of cycles and columns.
Pancharoen, Ura, "Separation technique on proteins via a pH-Parametric pump : a theoretical and experimental study" (1982). Dissertations. 1271.